The first batch of genetically modified rabbits was born in China's transgenic animal research

It was learned from the Dongying City Agricultural Research Institute in Shandong Province that the “Animal Breast Bioreactor” research project, a transgenic animal research project that was included in the national “863” project, has achieved important results in recent days. The GM rabbits produced by the research group were born one after another in local farms in January-February 2004. According to the latest test, among the 184 new born rabbits, 89 were positive individuals, and the positive rate reached 48.4%. Both the transgenic efficiency and the number of transgenic animals achieved major breakthroughs. This research was hosted by Deng Jixian, a researcher of the Chinese Academy of Military Medical Sciences, and related personnel from related scientific research and production units such as Shandong Laiyang Agricultural College and Dongying Tianyi Biotech Co., Ltd. jointly participated in the study. Animal mammary gland bioreactors are large-scale production of pharmaceutical proteins or other biologically active substances for the treatment and care of human diseases by preparing transgenic animals and using their mammary glands. Mammary glands of transgenic animals can replace bacteria and cells and other organisms for fermentation, and can effectively post-process and modify the produced protein. Therefore, animal mammary gland bioreactors are recognized internationally as one of the most promising directions in the research of transgenic animals, and are also an important way to realize the enticing prospects of genetic engineering pharmaceuticals. According to reports, in the current various methods for preparing transgenic animals, most of the techniques require expensive equipment and highly skilled technicians in the operation process, which results in large investment and long periods, which objectively limits the production of transgenic animals. Operability. Under this circumstance, the research group led by Deng Jixian designed different research routes and systematically studied the applications of somatic gene targeting, BAC vector method, and sperm carrier method in transgenic animals, and achieved certain research results. Among them, the research on sperm carrier method has made very valuable progress. Dr. Pan Qingjie and Dr. Shen Wei of the research group used the sperm vector method to prepare a transgenic rabbit model of mammary gland bioreactor. 184 transgenic rabbits of F1 generation were obtained. Through identification, 89 transgenic positive offspring were found, the positive rate was 48.4%. Among them, the newly invented DMSO method achieved a positive rate of 56.2% of transgenes.