"Three Measures" Breeding Quality Pleurotus ostreatus
The species of parent strains, original species and cultivars. It is also commonly referred to as first-class seed, secondary seed, and third-class seed. Its cultivation methods are different. Now the technical points are elaborated as follows:
1. Parent culture: Refers to the strains obtained by tissue separation or spore isolation. The mother culture medium is usually prepared using potato agar culture medium. Prepare 1000 ml of culture medium, 200 g potatoes, 18-20 g agar, and 20 g glucose.
Practice: Wash and peel potatoes first, cut into small pieces of broad bean, put 1000 ml of water and boil for 30 minutes, filter with 6-8 layers of gauze to make up for lost moisture. After the agar was added to dissolve, dissolved sugar was added and water was added to 1000 ml. Afterwards, aliquots of test tubes will be dispensed in a quantity of 1/5-1/4 per tube, plus tampon and wrapper. Sterilize under a pressure of 1.2 kg for 30 minutes and place it on a ramp. After cooling, the bacteria were inoculated under the conditions of 25°C-27°C. One female species can be piped 25-30.
Aseptically inoculated tubes are incubated at a constant temperature of approximately 25°C, and the mycelium can be grown in vitro in approximately half a month. During the cultivation process, care should be taken to remove the contaminated test tubes. The cultivated mother species is transferred to the refrigerator and stored at 4°C for six months or at room temperature for three months.
2, the original species culture: raw materials available wood chips or cottonseed skin. Sawdust medium formulation: wood chips 78%, wheat bran or rice bran 20%, sugar 1%, gypsum 1%. Cottonseed bark medium formulation: 50 kg of cottonseed meal plus potato juice 3%, (1.5 kilograms of potatoes cut into mud and boiled for 30 minutes of filtrate), sugar 0.1%, magnesium sulfate 0.1%.
Practice: Mix the material with water and mix it evenly. The humidity should be tightly grasped by hand. It is advisable to see drops of water in the fingers and it is not appropriate to drop them. The general ratio of water to water is 1:1.2-1.3. Then bottling, the bottling method is to gently shake the bottle while loading, so that the upper and lower parts of the bottle are close to the middle, and the shoulders of the bottle will be flattened, the culture materials inside and outside the bottle mouth will be cleaned, and the tampon is wrapped in paper. It is put into the autoclave for sterilization and sterilized under a pressure of 1.5 kg for one hour. After cooling, inoculate under sterile operating conditions. The parent species in the test tube is inserted into the bottle, and one parent species can receive 4-5 bottles of the original species. After inoculation, the mycelium is incubated at a constant temperature of 25°C for about one month to grow the bottle, which is the original species.
3. Cultivation of cultivars: The preparation of cultivars is actually an expansion of the original species. The formulation of the cultivating seed wood culture medium is the same as that of the original seed culture. Cottonseed bark medium formula is also the same as the original culture formula. Bacteria are sterilized after bottling, and the original species of bacteria is inserted into the cultivating species bottle under aseptic conditions. A bottle of 750 ml of the original species can be connected to 80-100 bottles of cultivars. After inoculation, it is incubated at a constant temperature of 25°C for about one month, and it can be sown after being filled with the bottle. When cultivating primary species and cultivated species, inspect once every 2-3 days after inoculation until the mycelium is over the material surface and check once a week. Pick out bacterial strains that have been contaminated with bacteria and treat them away from the culture room.
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