The simple method of sterilizing wet tampon with edible fungi

The sterilization equipment used for this technique is the “Supor” pressure cooker, which is the pressure cooker for home cooking, also known as the domestic pressure cooker. The container containing the mother culture medium is a colorless glass test tube of 18180 mm or 20200 mm; the container containing the original culture medium is a 250 ml or 500 ml infusion bottle, that is, a physiological saline bottle.
Sterilization operation: Inject water into the “Supor” pressure cooker by 5 cm deep, and prepare the prepared mother mushroom and original culture medium into glass test tubes and infusion bottles respectively according to the conventional method. The cotton plug plugs the sealed test tube mouth and the infusion bottle. mouth. After loading, the parent or original culture medium is directly erected in a pressure cooker, covered with a lid, and sterilized by fire for 40 minutes to 50 minutes.
The technique breaks the traditional method that needs to use kraft paper to wrap tube mouths or bottle mouth cotton plugs. The whole blockade autoclave but still can not guarantee the problem of 100% wet cotton plugs, achieves no special autoclave, no advanced sterilization equipment The ideal effect of non-wrapping cotton plugs that are fully sterilized but 100% non-wet cotton plugs. The outstanding feature is that the sterilization equipment is a domestic cooking utensil, which can be used in one pot, and is inexpensive and easy to purchase. The sterilization method is easy to apply and the sterilization effect is good. Completely solve the difficult problem of difficult production and sterilization of the parent species and original species of edible fungi, and open up a new world for simple production of edible fungi.

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