The fourth method of vitamin determination for pre-treatment solutions for food safety testing
/// Determination of vitamins A, D, and E in food (fourth method)
The GB 5009.82-2016 “ Measurement of Vitamins A, D and E in Foods †issued by the National Health and Family Planning Commission of the People's Republic of China and the State Food and Drug Administration on December 23, 2016 was officially implemented on June 23, 2017. .
The first method of the new standard applies to the determination of vitamin A and vitamin E in food ; the second method applies to the determination of vitamin E in foods such as edible oil, nuts, beans and paprika ; the third method applies to vitamin D2 in foods and Determination of vitamin D3 ; the fourth method is applicable to the determination of vitamin D2 or vitamin D3 in formula foods .
Then we know that the food sample needs to be pre-processed before the analysis and testing, and the sample pre-processing time may take up 60% of the entire detection process, and the final result error of 50% may be derived from the pre-treatment process. So, for the new standard, how can IKA's pre-processing equipment help experimenters improve the efficiency and accuracy of pre-processing? Let us taste the IKA pre-processing solution by interpreting the new standards.
Determination of vitamin D in foods of the fourth method - High performance liquid chromatography
experiment process
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Sample Preparation -> Sample Processing -> Extraction
Wash-> concentration- > sample determination
GB 5009.82-2016 requirements for sample preparation:
1.1 Sample preparation: After the sample has been shrunk, pulverized or homogenized, it is stored in the dark.
Tube mill 100 tube mill
Can be equipped with multiple grinding cups for grinding of batch samples
Program control to ensure repeatability of different batches of samples
T25 digital dispersion machine
Suitable for homogenization of different types of samples such as vegetables, fruits and meat
Special zigzag cutter head to solve the problem of sample winding
1.2 Sample treatment: Take 5~10g solid sample or 50g liquid sample in 150ml flat-bottomed flask --> add internal standard solution--> (including starch solution, add amylase, shake in a constant temperature water bath for 60min at 60 °C ) - -> addition of ascorbic acid and BHT mixing -> absolute ethanol and potassium hydroxide solution, shaken plus side -> 80 deg.] C to the reflux temperature of saponification magnetic stirrer 30min -> cooled to room temperature in cold water
Note: If the saponification solution is cooled, there is oil slick on the liquid surface, and the saponification time should be extended appropriately.
ICC RO 15 multi-point magnetic stirring constant temperature water bath
RT~100°C
Perfectly achieve mixing and mixing with water bath temperature control, mixing more powerful, more precise temperature control, optimize saponification process
Special bath design, liquid level height 32-72mm, avoiding small sample floating
Saponification of 15 samples at a time
Water bath and multi-point magnetic stirrer can be used independently
RO 15 multi-point magnetic stirrer
Achieve liquid mixing of batch samples
Positive and negative reversal function to increase the dissolution of insoluble samples
Closed design to avoid solution penetration
1.3 Extraction: Transfer the saponification solution into a 250 ml separatory funnel --> Add petroleum ether - ether to shake for 5 min --> Transfer the lower layer to another 250 ml separatory funnel --> Add the mixed ether solution and extract again
HS 501 reciprocating oscillating shaker
Batch extraction, AS501.2 can hold 6 250ml separatory funnels at a time
Timing function to ensure repeatability
Weight up to 26KG to avoid drifting during oscillation
A variety of fixtures are available to suit different applications
1.4 Concentration: The washed ether layer was filtered into a 250 ml rotary evaporation flask, and distilled under reduced pressure to a 2 ml ether solution on a 40 ° C water bath. The evaporation flask was removed and blown to near dryness with nitrogen. Ning hexane to 2ml, purify the test solution
RV10 auto control rotary evaporator patent - quantitative distillation
Accurate quantitative concentration to avoid evaporation
Batch sample concentration
About IKA
IKA Group is the market leader in pre-laboratory, calorimetric analysis, mixed dispersion industrial technology. Magnetic stirrers, overhead stirrers, dispersion homogenizers, mixers, constant temperature shakers, grinders, rotary evaporators, Heating plates, constant temperature circulators, calorimeters, laboratory reactors, photobioreactors and other related products constitute the product line of IKA laboratory analysis, while industrial technology mainly includes mixing equipment for scale production, dispersing emulsification equipment, Kneading equipment, and a complete solution from pilot to expanded production. The group is headquartered in Staufen, southern Germany, with branches in the United States, China, India, Malaysia, Japan, Brazil, the United Kingdom, Poland and other countries.
Founded in 1910, IKA is now proud to review the history of the past 100 years.
Edible mushroom polysaccharide;Wolfberry polysaccharide;Jujube polysaccharide
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