Precautions for gel imaging gel cutting operation
1. The electrophoresis buffer and gel are all made freshly. The table for cutting the glue is cleaned, and the blade is best cleaned and sterilized. One of the words is to ensure that the cut tape has no foreign DNA pollution.
2. The glue is to recover all the glue in the position of the entire target piece. In order to reduce the volume of the glue, it can be done with a relatively thin glue, as long as it is enough to be spotted, a thin and wide comb can also be used to run the glue.
3. Regarding the protection, it is enough after the general plexiglass, put on a protective mask to protect the eyes, if you wear resin glasses. If you are very afraid of UV radiation, or have special requirements for glue, such as the requirement for EB-free, you can use a dot marker and a graduated ruler to take a photo to determine the position of the target strip on the glue.
4. Follow the normal procedure to mark the glue, then cut the glue with the marker, EB stain, and take photos with the graduated ruler under the UV lamp. Since the relative position between the belt to be recovered and the marker is known, the position of the target belt on the undyed glue can be measured by the ruler. If you find it difficult to judge, you can point a small amount directly on the marker, so the position is easy to determine.
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