Introduction and purchase of cell bottle transfer machine

There are a variety of classification methods and types of cell transfer machines, which can be classified according to the culture method, rotation speed, volume of the bottle, and number of bottles. Users need to make reasonable choices according to their needs.

(1) Training method

According to the culture method, the cell bottle transfer machine can be divided into suspension culture, adherent culture, and adherent/suspension culture in one.

(2) Rotating speed

Different types of cell turners have different speeds. Under normal circumstances, the cell-rolling machine for adherent culture has an inlet speed of 3-60 rpm, and domestically produced 3-20 rpm. The rotational speed of the inlet of the cell culture bottle in suspension culture is 60-900 rpm, and the rotation speed of the two-in-one of the adherent/suspended culture is generally 1-999 rpm.

(3) Rotating bottle volume

There are several different types of bottles: 1500ml, 2000ml, 3000ml, 10000ml, 15000ml.

(4) Number of bottles

Generally, the cell bottle transfer machine is divided into several layers. The total number of bottle bottles is an important parameter for the user when selecting the bottle-turning machine: usually the volume of the bottle of the 1500ml bottle has 2, 3, 4, 6, 8; 2000ml. The number of bottles corresponding to the volume of the bottle is 4,8; the number of bottles corresponding to the volume of the bottle of 3000ml is 4-80; the number of bottles corresponding to the volume of the bottle of 10000ml is 20,25; the bottle of 15000ml The number of bottles corresponding to the volume varies from 20 to 30.

The installation and use of the cell bottle transfer machine is relatively simple, no need for engineers to install and debug.

Cell Culture Bottle Transfer Machine (Cell Reactor) Selection Guide:

The selected cell culture instruments are not the same according to the experimental purposes of different laboratories. The following recommendations are made for years of instrument sales and usage:

1. Small laboratory mammalian cells are cultured in a magnified manner, and the cultured subject (animal cells) is selected in the case of little influence on the shearing force, and the cell culture is a bottle-turning machine. Mainly used for suspension and adherent culture of cells. (Select domestically produced 2-10 bottle cell culture transfer bottles to meet the needs, the culture conditions can be selected together with different incubators, the price of the bottle-turning machine is 5000-15000 yuan; if the research funding is sufficient, please choose The imported CELLROLL bottle spinning machine with imported, rotating bottles and incubators, the culture system is stable, and the price is in RMB.

2. Small laboratory mammalian cells, insect cell culture, used for the culture of suspension cells and adherent cells, ideally used in microcarrier cell culture, biomixer. (It is recommended to use the Swiss Cellpin cell bottle transfer machine, which provides the compact Spinner system with double-clock hammer mixing, which minimizes the shear force and the price is 50,000-70,000 RMB).

3. Cultivation of small animal cells, growth of insect cells and plant cells; culture of shear-sensitive cells and anaerobic bacteria; selection of transition cultures for culture from shakers or tissue culture flasks, bag cell culture incubators. (Bag type cell culture device, although the shearing force is reduced a lot, but the uniformity of the concentration of the culture system is not very good, so do not use it under special circumstances. Under normal circumstances, the Swiss Cellspin cell bottle vending machine has been used to meet the needs. The needs of most culture systems).

4, large-scale cell culture, cell culture, bio-metabolite production production enterprises choose, multi-functional bioreactor. There are many types of large-scale bioreactors, which can be divided into animal adherent cell culture reactors, animal cell adherent culture bioreactors, insect cell bioreactors, and bacteria according to user requirements, that is, culture objects. Biological fermentation bioreactor.