Analysis of the idea of ​​circulating RNA in rectal cancer metastasis with a factor of up to 7.8
Article introduction:
In a large family of non-coding RNAs, circular RNA is another star member following microRNAs and lncRNAs in recent years. Because of its looping at the 3'-5' end, it has high stability and is not easily degraded in organisms. Most of the circular RNA participates in the sponge mechanism by directly or indirectly regulating miRNA and target genes. Several articles have reported that this mechanism plays a crucial role in the occurrence and development of diseases. In addition, circular RNA is usually differentially expressed in diseased tissues and, therefore, is well suited as a molecular marker for cancer. At present, there is no relevant report on the field of rectal cancer metastasis. In this paper, we used circular RNA sequencing to compare the rectal cancer patients (CRC) and rectal cancer metastasis to liver cancer patients (CRC-m) tissue samples, and successfully constructed the first circular RNA expression profile of rectal cancer in China. By expanding the sample size, two differentially up-regulated circular RNAs, circRNA_0001178 and circRNA_0000826, were confirmed, which have clinical value as molecular markers. Finally, a circular RNA-miRNA-mRNA network map was constructed to visually reveal the potential relationships among the three.
Journal: Molecular Cancer
Impact factor: 7.8
Service content: Circular RNA sequencing (provided by Cloud Sequence Bio )
Published: January 2019 Sequencing samples: 1. Rectal cancer patients (CRC) and rectal cancer metastasis to liver cancer patients (CRC-m) cancer tissue, sample size: 3 vs 3
Validation samples: 2. Rectal cancer patients (CRC) and rectal cancer metastasis to liver cancer patients (CRC-m) cancer tissue, sample size: 24 vs 16
Article content:
1. Complete transcriptome sequencing to construct circular RNA expression profiles <br> The high-throughput sequencing technology was used to detect the expression of circular RNA in cancer tissues of CRC and CRC-m patients, and 66,855 circular RNAs were detected. The difference is ≥ 2 times, P value ≤ 0.05), 92 of which are differentially up-regulated, and 21 of the circular RNA are down-regulated. The differentially expressed circular RNAs are mostly exon-looped and may have the ability to encode proteins. At the same time, they are distributed on other chromosomes except that they are not distributed on the Y chromosome.
In general, this article first sequenced rectal cancer metastatic liver cancer and rectal cancer samples to construct a circular RNA expression profile. From the sequencing results, four circular RNAs with the most obvious difference were screened. Two low-throughput circular RNAs were obtained by low-throughput validation of clinical samples. Finally, a miRNA and a target gene capable of binding to it are predicted to construct a circular RNA-miRNA-mRNA network map.
Full text link:
Https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-018-0932-8
Cloud order related product recommendation:
Whole transcriptome sequencing
Circular RNA sequencing
LncRNA sequencing
Circular RNA quantitative PCR
M6A RNA whole transcriptome methylation sequencing
M5C RNA whole transcriptome methylation sequencing
m1A RNA whole transcriptome methylation sequencing
RNA pull down
RIP sequencing
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