Optimization of culture conditions for selenium-enriched plant bacillus
Selenium is one of the 14 trace elements necessary for the human body, and it has important physiological functions such as participating in immune regulation, preventing cancer, and inhibiting pathogenic bacteria. Selenium is also an essential component of many enzyme activity centers in mammals, such as glutathione peroxidase (GPx), deiodinase (ID), etc., which protect cells from excessive oxidative damage.
Studies have shown that cereals, fruits and vegetables have low levels of selenium and do not meet the daily dietary needs of selenium 55 μg/d (recommended dietary intake). The common inorganic selenium selenite has strong toxic side effects. Therefore, the method of converting inorganic selenium into intracellular organic selenium through the absorption and metabolism process of the microbes as a carrier has received extensive attention from relevant experts at home and abroad.
As a new source of organic selenium, lactic acid bacteria have been recognized by consumers because of its good health care function and reliable safety. Its biological effects and potential development and application prospects are very broad. In this paper, the selenium-enriching ability experiment was carried out with the induced Lactobacillus plantarum as the starting strain. Firstly, the method for determining the selenium-enriched amount of the bacteria was introduced. Then the best selenium-enriched strain of Lactobacillus plantarum was determined by single factor experiment and corresponding surface analysis. The culture conditions lay the foundation for further research and application of selenium-enriched lactic acid bacteria.
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