How to measure the enzyme activity after blood collection?
Enzyme catalytic activity has gone from high-cold biology textbooks to people's side - it is often seen in medical reports, as an important indicator of its health, it is very common in clinical practice. Test items.
However, since the enzyme catalytic activity is determined not only by the enzyme content, but also by various factors, such as the nature and concentration of the substrate used, the pH of the reaction medium, temperature, ionic strength, activation or inhibition factor, etc., it is method dependent. . This is a major problem in determining the catalytic activity of the enzyme.
There are many reference ranges for the same enzyme project, and the results between the laboratories are not comparable. The target value of the sample for inter-room quality evaluation is difficult to determine, and sometimes it may cause clinical misdiagnosis.
Therefore, research on enzyme catalytic activity in recent years has been searching for a faster and more accurate method.
Today, Xiao Ai wants to discuss with you the method of using the spectrum to achieve.
Taking the classical chemical color change reaction as an example, there is also a change in absorbance which occurs with the reaction of the substrate in the reaction kinetics of the enzyme. In the conventional mode, after the end of the enzyme reaction, the absorbance of the experimental group and the control group are sequentially measured, which may cause errors due to the operation time. If the spectrometer is used to acquire the absorbance curve in different bands, it is faster, more accurate and easy to reproduce.
In the process, IKA Germany can provide you with a stable magnetic stirrer, allowing you to focus on the work of the spectrometer without worrying about the homogeneity of the enzyme reaction.
If your spectrometer has high background requirements and needs to be protected from light, the compact and powerful Topolino can be easily placed in a black box, and the Topolino mobil (portable rechargeable model) reduces the power cord for you to operate in the dark box. It's more convenient, no need to worry about the power cord need to extend outside the box or inadvertently touch the reaction vessel.
The Topolio series of IKA in Germany pays attention to your experiments in detail, and the humanized design makes you love it.
About IKA ( )
IKA Group is the market leader in pre-laboratory, calorimetric analysis, mixed dispersion industrial technology. Magnetic stirrers, overhead stirrers, dispersion homogenizers, mixers, constant temperature shakers, grinders, rotary evaporators, Heating plates, calorimeters, laboratory reactors and other related products constitute the product line of IKA laboratory analysis, while industrial technology mainly includes mixing equipment for scale production, dispersion emulsification equipment, kneading equipment, and from pilot to expansion. The company's headquarters is located in Staufen, southern Germany. It has branches in the United States, China, India, Malaysia, Japan, Brazil and other countries. IKA was founded in 1910, and IKA Group can now proudly look back over the past 100 years. history.
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